Monday, September 27, 2010

How to design and form a DNA microarray chip?

There are a number of different technology for forming DNA microarrays.
In all of them, a tiny drop of juice, containing a target DNA, is deposited on a surface (usually glass or a plastic-like compound) within a precise pattern which forms the array.
Getting the amount of DNA to be surely reproducible is a trick and takes a large amount of work. That's where profoundly of the technology is focused. Generally, the best devices use a robotic system in a humidity- and temperature-controlled chamber.
You also want to have several different kinds of source DNA for use as a target. That requires keep of thousands and thousands of clones.
I dont know
There are two different types of DNA microarrays widely in use at present, one made up entirely of short, synthetic oligonucleotides and others made up of strands of complementary DNA (cDNA). Each can be used successfully to blind for changes contained by gene expression. The critical features of their production is to have a lofty quality source of the DNA itself, and the other is to enjoy the technology to produce the slides onto which the samples are spotted. Robotic technology lower than highly controlled circumstances is most habitually used to produce the small droplets that are spotted in a gridded guide onto the slides.
Try going to the Affymetrix web site.we've used their chips contained by the lab with great results.

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