how somatotropin is produced using genetic engineering?

somatotropin is the growth hormone which helps within growth and metabolism. i want to know how it is manufactured via recombinant DNA technology? In short, the genetic sequence in DNA which codes for somatotropin proteins is isolated and cut from the mother strand by restriction enzymes. These cut strands of DNA are afterwards amplified or multiplied using a system called PCR (polymerase secure reaction). To check if they have the right one, they utilize a series of test usually called a blot which involves electrophoresis on an agar gel. When isolated, a host bacterium is special and the somatotropin DNA sequence is inserted into the bacterium's DNA ring via some vector and some more restriction enzymes. The sequence inserted in the microbes takes hold and the bacterium is fooled into thinking that the somatotroping sequence belongs at hand and begins producing the protien along near its other natural functions (this sector is the recombinant part). The somatotropin hormone is then harvest using diverse methods of separation and packaged for our use . . .
That's a short answer for a remarkably long process. You should take a semester of Molecular Biology and it will shed more pallid.
Fairly accurate answer below, but the industry is now moving toward creation of biomolecules w.o. live vectors, see Roche website, RNAs are used directly.

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